کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067105 | 1077882 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Smooth muscle cell differentiation from human bone marrow: Variations in cell type specific markers and Id gene expression in a new model of cell culture
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کلمات کلیدی
myocardin related transcription factorEGFSM-MHCMRTFSMCCalponinSRFbFGFGAPDHHuman - انسانDifferentiation - تفکیکSMA - دبیرستانSmooth muscle cell - سلول عضلانی صافSmooth muscle cells - سلول های عضله صافepidermal growth factor - عامل رشد اپیدرمیSmooth muscle α-actin - عضله صاف α-actinbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهserum response factor - فاکتور پاسخ سرمیBMP - مدیریت فرایند کسب و کارbone marrow - مغز استخوانBone morphogenetic protein - پروتئین مورفوژنیک استخوانId genes - ژنهای شناساییCaP - کلاه لبه دارglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
Stromal cells follow a vascular smooth muscle differentiation pathway. However, cell culture models performed from human bone marrow do not allow the obtention of a large proportion of highly differentiated smooth muscle cells (SMC) and their differentiation pathways remain unclear. We have characterized a new model of SMC differentiation from human bone marrow stromal cells by using different factors (bFGF, EGF, insulin and BMP-4). A relative homogeneous population of differentiated SMC was reproducibly obtained in short-term culture with high expression of SMC markers. Id gene expression was investigated and showed that (1) Id2 mRNA expression was upregulated during SMC differentiation without change of Id1 mRNA and (2) Id1 gene expression highly increased concomitantly with a decrease of SMC markers while Id2 mRNA was slightly modulated. Our data suggested that Id genes are potentially implicated in the differentiation pathway of human SMC from bone marrow.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 33, Issue 6, June 2009, Pages 621-631
Journal: Cell Biology International - Volume 33, Issue 6, June 2009, Pages 621-631
نویسندگان
Karine Tréguer, François Naye, Pierre Thiébaud, Sandrine Fédou, Fabienne Soulet, Nadine Thézé, Corinne Faucheux,