کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067131 | 1077883 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro generation of murine myeloid dendritic cells from CD34-positive precursors
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
Dendritic cells (DCs) link the innate and adaptive immune system. Currently, murine DCs for cell biology investigations are developed from MHC class II-negative bone marrow (BM) precursor cells, non-depleted BM cells or BM monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Here we demonstrate an isolation procedure of functionally intact myeloid CD11c+ CD11b+ DCs derived from murine CD34-positive precursors. DCs derived from CD34+ cells show functional internalization, maturation, cytokine secretion, MHC-restricted antigen presentation, and MHCII retrograde transport of antigens from the lysosomes to the cell surface. In comparison to the established method, the advantages of this isolation procedure are a shorter cultivation period, a superior transfection efficiency, the yield of a purer and more homogeneous population of immature DCs, and less consumption of cell culture medium and GM-CSF. The new isolation procedure and the functional quality of CD34+ cell-derived murine myeloid DCs make them ideally suited for immunology and cell biology studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 33, Issue 7, July 2009, Pages 778-784
Journal: Cell Biology International - Volume 33, Issue 7, July 2009, Pages 778-784
نویسندگان
Tom-Li Stephen, Fabian Harms, Mario Fabri, Eva Flenner, Martina Bessler, Helena Hafke, Sonja Meemboor, Christoph Kalka, Wiltrud Kalka-Moll,