کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067292 | 1077891 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unique membrane-interacting properties of the immunostimulatory cationic peptide KLKL5KLK (KLK)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have monitored the effects of KLKL5KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation. Fractionation of vesicles revealed KLK predominantly in the membrane. Peptide-treated vesicles appeared with generally disorganized bilayers. While KLK showed no effect on osmotic resistance of human erythrocytes, dramatic decrease in core and surface membrane fluidity was observed in peptide-treated erythrocyte ghosts as measured by fluorescence anisotropy. Finally, CD spectroscopy revealed lipid-induced random coil to β-sheet and β-sheet to α-helix conformational transitions of KLK. Together with the oligonucleotide oligo-d(IC)13 [ODN1a], KLK functions as a novel adjuvant, termed IC31â¢. Among other immunological effects, KLK appears to facilitate the uptake and delivery of ODN1a into cellular compartments, but the nature of KLK's interaction with the cell surface and other membrane-bordered compartments remains unknown. Our results suggest a profound membrane interacting property of KLK that might contribute to the immunostimulatory activities of IC31â¢.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 32, Issue 11, November 2008, Pages 1449-1458
Journal: Cell Biology International - Volume 32, Issue 11, November 2008, Pages 1449-1458
نویسندگان
Michael C. Aichinger, Martina Ortbauer, Siegfried Reipert, Wolfgang Zauner, Péter Bogner, Elisabeth Froschauer, Karin Nowikovsky, Karen Lingnau, Alexander von Gabain, Rudolf Schweyen, Tamás Henics,