کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067368 | 1077895 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ca2+-dimethylphosphate complex formation: Providing insight into Ca2+-mediated local dehydration and membrane fusion in cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
Earlier studies using X-ray diffraction, light scattering, photon correlation spectroscopy, and atomic force microscopy, strongly suggest that SNARE-induced membrane fusion in cells proceeds as a result of calcium bridging opposing bilayers. The bridging of phospholipid heads groups in the opposing bilayers by calcium leads to the release of water from hydrated Ca2+ ions as well as the loosely coordinated water at PO-lipid head groups. Local dehydration of phospholipid head groups and the calcium, bridging opposing bilayers, then leads to destabilization of the lipid bilayers and membrane fusion. This hypothesis was tested in the current study by atomistic molecular dynamic simulations in the isobaric-isothermal ensemble using hydrated dimethylphosphate anions (DMPâ) and calcium cations. Results from the study demonstrate, formation of DMP-Ca2+ complexes and the consequent removal of water, supporting the hypothesis. Our study further demonstrates that as a result of Ca2+-DMP self-assembly, the distance between anionic oxygens between the two DMP molecules is reduced to 2.92Â Ã
, which is in close agreement with the 2.8Â Ã
SNARE-induced apposition established between opposing bilayers, reported earlier from X-ray diffraction measurements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 32, Issue 4, April 2008, Pages 361-366
Journal: Cell Biology International - Volume 32, Issue 4, April 2008, Pages 361-366
نویسندگان
Jeffrey J. Potoff, Zeena Issa, Charles W. Jr., Bhanu P. Jena,