کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067522 | 1077898 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of nicotine and polyaromtic hydrocarbons on cerebral endothelial cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
The present study was designed to investigate the effect of nicotine and polyaromatic hydrocarbon compounds on cerebral endothelial cells (CECs). Nicotine treatments from 15Â min to 5Â h did not cause any changes in the expression and localization of principal junctional proteins. One day of treatment with a relatively high concentration of nicotine induced a decrease in the expression of the tight junction protein ZO-1, occludin, and the adherens junction protein, cadherin. Treatment with 3Â ÃÂ 10â5Â M phenanthrene for 24Â h caused a redistribution of occludin from the Triton X-100 insoluble to the Triton X-100 soluble fraction. Transendothelial electrical resistance was not significantly affected by 24Â h treatments with nicotine, methylanthracene or phenanthrene. However, 24Â h nicotine treatment increased transendothelial permeability in CECs exposed to oxidative stress. Both nicotine and phenanthrene were able to regulate the expression of a large number of proteins as revealed by 2D electrophoresis. Our experiments suggest that tobacco smoking may affect the junctional complex of CECs, and that this effect is enhanced by oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 32, Issue 2, February 2008, Pages 198-209
Journal: Cell Biology International - Volume 32, Issue 2, February 2008, Pages 198-209
نویسندگان
Pilaiwanwadee Hutamekalin, Attila E. Farkas, Anna Orbók, Imola Wilhelm, Péter NagyÅszi, Szilvia Veszelka, Mária A. Deli, Krisztina Buzás, Ãva Hunyadi-Gulyás, Katalin F. Medzihradszky, Duangdeun Meksuriyen, István A. Krizbai,