کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067622 | 1077902 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Colocalization of aldolase and FBPase in cytoplasm and nucleus of cardiomyocytes
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کلمات کلیدی
HEPESIntercalated discsFBPaseN-[2-hydroxyethyl] piperazine-N′-[2-ethanesulfonic acid]WGATRITCNLSTBSEGTAFITCethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیس (β-آمینویل اتر) -N، N، N '، N'-tetraacetic اسیدtetramethylrhodamine isothiocyanate - تترامتییل رودامین ایزوتیوسیاناتTris-buffered saline - تریس بافر شورnuclear localisation sequence - توالی محلی سازی هسته ایfructose 1,6-bisphosphatase - فروکتوز 1،6-بیسفسفاتازfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتHeterochromatin - هتروکروماتینNuclear import - واردات هسته ایGlyconeogenesis - گلیکوژنزWheat germ agglutinin - گیاه گندم آگلوتیینین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The protein exchange method, immunocytochemistry and the nuclear import of fluorophore-labeled enzymes were used to investigate the colocalisation of aldolase and FBPase in cardiomyocytes. The results indicate in vivo interaction of these two enzymes. In the cardiomyocyte cytoplasm, these enzymes were found to colocalise at the Z-line and on intercalated discs. The translocation of both enzymes through the nuclear pores was also investigated. The immunocytochemistry revealed the colocalisation of aldolase and FBPase in the heterochromatin region of cardiomyocyte nuclei. The Pearson's correlation coefficients, which represent the degree of colocalisation were 0.47, 0.52 and 0.66 in the sarcomer, the intercalated disc and the nucleus, respectively. This is the first report on aldolase and FBPase colocalisation in cardiomyocytes. Interaction of aldolase with FBPase, which results in heterologous complex formation, is necessary for glyconeogenesis to proceed. Therefore, this metabolic pathway in the sarcomer, in the intercalated disc as well as in the nucleus might be expected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 10, October 2007, Pages 1122-1130
Journal: Cell Biology International - Volume 31, Issue 10, October 2007, Pages 1122-1130
نویسندگان
Piotr Mamczur, Danuta Dus, Andrzej Dzugaj,