کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2067717 1077906 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Silencing invariant chain of DCs enhances Th1 response using small interfering RNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Silencing invariant chain of DCs enhances Th1 response using small interfering RNA
چکیده انگلیسی
RNA interference (RNAi), which causes the degradation of any RNA in a sequence specific manner, is a posttranscriptional gene silencing mechanism. Targeting the invariant chain (Ii) in DCs has been used as an approach to enhance antitumor immunity. It is demonstrated in this article that transfection of H-2K DCs with siRNA specific for Ii gene can significantly knock down Ii. When exposed to TNF-α, immature DCs transfected with Ii siRNA can differentiate into mature DCs without reducing viability or IL-12p70 production. Ii siRNA-treated H-2K DCs exhibited an increased allostimulatory capacity in a lymphocyte proliferation assay. Furthermore, Ii siRNA-transfected H-2K DCs enhanced Th1 responses by increasing IFN-γ and decreasing IL-4 production, and much stronger cytotoxic activity was observed when DCs were co-transfected with Ii siRNA and an endogenous tumor antigen in vitro. Our findings indicate that silencing the Ii gene in DCs with siRNA may offer a potential approach to enhancing antitumor immunotherapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 7, July 2007, Pages 663-671
نویسندگان
, , , , , , ,