کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067742 | 1077907 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunohistochemical study of flotillin-1 in rat testis with ischemia/reperfusion injury
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کلمات کلیدی
FITCTRITCFlotillin-1tetramethyl rhodamine isothiocyanateI/R - I / Rischemia and reperfusion - ایسکمی و رپرفیوژنTestis - بیضهTesticular torsion - تورفتگی مفصلیSertoli cell - سلول سرتولیfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMacrophage - ماکروفاژ Nitric oxide - نیتریک اکسیدcathepsin D - کاتهپسین D
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To investigate the involvement of flotillin-1 in acute experimental testicular torsion, we examined the expression and cellular localization of flotillin-1 and cathepsin D in the rat testis with ischemia/reperfusion (I/R) injury. Western blot analysis showed that the expression of flotillin-1 increased significantly 6Â h after I/R and that the level remained elevated for 48Â h. Immunohistochemically, flotillin-1 was constitutively localized in some Sertoli cells, peritubular myoid cells, and interstitial cells in the normal testis. After I/R injury, Sertoli cells in the damaged tubules were intensely immunostained for flotillin-1 at 24 and 48Â h after I/R. Flotillin-1 was also detected in some inflammatory cells in the interstitial space around damaged tubules. Furthermore, flotillin-1 was colocalized with cathepsin D, a lysosomal marker, in normal testis (mainly in Sertoli cells), and the colocalization was greater in Sertoli cells and macrophages in I/R injured testes. Therefore, we postulate that flotillin-1 immunoreactivity is increased in some Sertoli and inflammatory cells (especially in ED1-positive activated macrophages) in testicular torsion and that flotillin-1 in the injured testis associates with lysosomes in Sertoli cells and macrophages, activating subsequent signals in inflammatory macrophages and Sertoli cells after I/R.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 6, June 2007, Pages 609-614
Journal: Cell Biology International - Volume 31, Issue 6, June 2007, Pages 609-614
نویسندگان
Chan-woo Jeong, Heechul Kim, Seungjoon Kim, Sung-Ho Kim, Changjong Moon, Taekyun Shin,