کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067860 | 1077914 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of muscarinic acetylcholine receptors in chloride secretion by cultured rat epididymal epithelium
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Involvement of muscarinic acetylcholine receptors in chloride secretion by cultured rat epididymal epithelium Involvement of muscarinic acetylcholine receptors in chloride secretion by cultured rat epididymal epithelium](/preview/png/2067860.png)
چکیده انگلیسی
The aim of our present study was to investigate the short-circuit current response to carbachol in cultured rat cauda epididymal epithelia and the signal transduction mechanisms involved. Carbachol added basolaterally induced a concentration-dependent increase in short-circuit current (Isc) across the epididymal epithelium consisting of a rapidly rising phase and a long term sustained response. The response was almost abolished by removing Clâ from the extracellular medium and blockable by pretreating the tissues with DPC, indicating a substantial contribution of Clâ secretion to the carbachol-induced response. The muscarinic acetylcholine receptor antagonist atropine inhibited the response, but the nicotinic acetylcholine receptors antagonist curarine had no effect, suggesting that only the muscarinic acetylcholine receptors mediated the secretory response of the basolateral side of rat cauda epididymal epithelium to carbachol. Addition of carbachol to the apical side of the tissue was found not to elicit an Isc response. These results suggested that muscarinic receptors are present in the basolateral side of rat cauda epididymal epithelium. Activation of these receptors by acetylcholine released from the nerve endings regulates epididymal transepithelial Clâ secretion. Cholinergic stimulation therefore contributes to the formation of luminal fluid microenvironment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 9, September 2006, Pages 741-746
Journal: Cell Biology International - Volume 30, Issue 9, September 2006, Pages 741-746
نویسندگان
Jian-Yang Du, Wu-Lin Zuo, Min-Hui Chen, Hui Xiang, Wen-Liang Zhou,