کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067880 | 1077915 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improved Taxol production by combination of inducing factors in suspension cell culture of Taxus baccata
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
For morphological analysis, microscopic inspection was carried out during cultivation. Cell-associated and extracellular amount of Taxol were detected and measured using HPLC methodology. At stage I, overall Taxol amount of biomass growth medium was 13.75Â mg/l (i.e., 5.6-fold higher than that of untreated B5 control). At stage II, treated cells with methyl jasmonate (10Â mg/l), salicylic acid (100Â mg/l) and fungal elicitor (25Â mg/l) produced the highest amount of Taxol (39.5Â mg/l), which is 16-fold higher than that of untreated B5 control (2.45Â mg/l). Microscopic analyses of Taxus cells in suspension cultures showed various positional auto-fluorescence showing direct correlation with Taxol production. Our studies revealed that intervallic supplementation of B5 medium with combination of biomass growth factors at stage I and mixture of elicitors at stage II could significantly increase Taxol production. Thus, we suggest that the exploitation of this methodology may improve the production of Taxol since demands for Taxol pharmaceuticals are increasingly growing and resource paucities have limited its direct harvesting from Taxus trees.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 3, March 2006, Pages 262-269
Journal: Cell Biology International - Volume 30, Issue 3, March 2006, Pages 262-269
نویسندگان
A. Yari Khosroushahi, M. Valizadeh, A. Ghasempour, M. Khosrowshahli, H. Naghdibadi, M.R. Dadpour, Y. Omidi,