کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067915 | 1077918 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activity of Pichia pastoris alternative cis-prenyltransferase is correlated with proliferation of peroxisomes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have investigated dolichol synthesis in yeast Pichia pastoris. Growth of these cells on methanol causes peroxisome proliferation and induction of peroxisomal enzymes. Twenty-four hours methanol treatment was sufficient for the appearance of longer-chain dolichols. Less specific oleic acid induction needed 48Â h for the synthesis of longer dolichol family with typical one still present. Cells cultured in non-inducing conditions for 48Â h did not reveal the presence of additional dolichol family. Peroxisomes purified from oleic acid treated cells synthesize in vitro polyprenols longer by two isoprene residues than those synthesized by microsomal fraction from glucose culture. These observations lead us to suggest that chain length of dolichols synthesized in yeast cell may depend on the carbon and energy source supply which mobilizes metabolic pathways localized to different cellular compartments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 2, February 2006, Pages 122-126
Journal: Cell Biology International - Volume 30, Issue 2, February 2006, Pages 122-126
نویسندگان
Marek Skoneczny, Barbara Kludkiewicz, Ewa Swiezewska, Anna Szkopinska,