کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2069128 | 1078374 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Wy-14,643 and fenofibrate inhibit mitochondrial respiration in isolated rat cardiac mitochondria Wy-14,643 and fenofibrate inhibit mitochondrial respiration in isolated rat cardiac mitochondria](/preview/png/2069128.png)
We investigated the direct effects of two selective PPARα ligands, fenofibrate and Wy-14,643, on mitochondrial respiratory function using isolated rat cardiac mitochondria. Isolated left ventricular mitochondria were incubated with increasing concentrations of fenofibrate or Wy-14,643 (10, 100, and 500 μM) and mitochondrial respiration determined using: malate/glutamate (complex I), succinate (complex II) and palmitoyl-l-carnitine as oxidative substrates. Our data show that acute exposure to Wy-14,643 and fenofibrate differentially perturb cardiac mitochondrial respiration i.e., fenofibrate more potently inhibited mitochondrial respiration and bioenergetic capacity compared to Wy-14,643. Moreover, we found that both agents increased uncoupling of mitochondrial oxidative phosphorylation.
Journal: Mitochondrion - Volume 6, Issue 6, December 2006, Pages 315–322