کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2072631 | 1544718 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Natriuretic peptides stimulate oocyte meiotic resumption in bovine
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Natriuretic peptides stimulate oocyte meiotic resumption in bovine Natriuretic peptides stimulate oocyte meiotic resumption in bovine](/preview/png/2072631.png)
چکیده انگلیسی
The aim of the present study was to evaluate the expression of mRNA encoding natriuretic peptides (NPs) and their receptors in the cumulus-oocyte complex in cattle, a monovular mammalian species, and also to investigate the role of NPs in oocyte meiotic resumption in vitro. mRNA was observed for the NP precursor type-A (NPPA), type-C (NPPC), NP receptor-1 (NPR-1), receptor-2 (NPR-2) and receptor-3 (NPR-3) in bovine cumulus cells, and NPR-2 mRNA was observed in oocytes. These results are different from those obtained in mouse and pig models. The effects of NPPA, NP precursor type-B (NPPB) and NPPC on the resumption of arrested meiosis maintained by forskolin were studied at three different doses (10, 100 and 1000 nM) with a 12 h culture system. The germinal vesicle breakdown rates were greater (P â¤Â 0.05) in oocytes that were cultured in the presence of one or a combination of NPs (from 44% to 73%) than the negative control (from 24% to 27%). Additionally, it was demonstrated that the concentration of cyclic guanosine 3â²,5â²-monophosphate (cGMP) is increased by NPPA and NPPC in oocytes and cumulus cells after 3 h of in vitro maturation. However, in both groups, the concentration of cyclic adenosine 3â²,5â²-monophosphate (cAMP) in the oocyte did not increase between 3 and 6 h of culture, even when forskolin was used. In summary, we observed the presence of mRNA for NPs and their receptors in the bovine cumulus-oocyte complex and demonstrated that, in vitro, NPPA, NPPB and NPPC stimulate oocyte meiotic resumption in a monovular species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 159, August 2015, Pages 52-59
Journal: Animal Reproduction Science - Volume 159, August 2015, Pages 52-59
نویسندگان
Matheus P De Cesaro, Mariana P. Macedo, Joabel T. Santos, Paulo R.A Rosa, Charles A. Ludke, Vitor B. Rissi, Bernardo G. Gasperin, Paulo B.D Gonçalves,