کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2072424 1544705 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip
ترجمه فارسی عنوان
جداسازی اسپرم‌های متحرک برای لقاح در شرایط آزمایشگاهی از منی منجمد ـ ذوب شده گاو نر با استفاده از القای پروژسترون در یک ریزتراشه
کلمات کلیدی
ریزتراشه؛ القای پروژسترون؛ جدایی؛ اسپرمهای متحرک؛ میتوکندری IVF
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

This study presents a novel method for the separation of motile sperm from non-progressive motile and immotile sperm and in vitro Fertilization (IVF). This separation of bull sperm was accomplished by inducing chemotaxis along a progesterone release agent in a 7.5-mm microchannel microchip composed of a biocompatible polydimethysiloxane layer and a glass gradient. The selected sperm was applied directly for IVF. In the first experiment, we tested the effect of different lengths of microchannnel (5 mm, 7.5 mm and 10 mm) on quality parameter of separated sperm. The results showed that separated sperm using 7.5-mm microchannel chip were improved in sperm motility, swimming velocity, and beat frequency compared with other groups. In the second experiment, a medium containing sperm from swim-up method and outlet reservoir of our 7.5-mm microchannel chip was collected and mitochondrial activity of the sperm was determined by fluorescence microscopy. The sperm from the microchip had higher mitochondria activity (47.6% ± 6.0%) than the sperm from the swim-up method (23.6% ± 4.7%) (P < 0.05). There were significant differences in rate of acrosome intactness between the swim-up method and the microchip (36.0% ± 4.1% vs. 66.8 ± 2.1%, respectively, P < 0.05). In the third experiment, we compared sperm penetration in the microchip-IVF system with a standard IVF method (droplet-IVF). The microchip-IVF group had the highest percentages of oocytes penetrated (82.2% ± 1.6% vs. 63.5% ± 2.4%) and monospermic oocytes (67.8% ± 3.4% vs. 42.4% ± 1.5%). In addition, early developmental competence of oocytes to the blastocyst stage was higher when the oocytes were inseminated in the microchip-IVF system compared with those inseminated in a standard droplet-IVF system. These results demonstrate that our microchip based on a sperm chemotaxis system is useful for motile sperm separation from frozen-thawed bull semen for IVF. Therefore, the optimized microchip system provides a good opportunity to sort motile bull sperm for IVF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 172, September 2016, Pages 52–59
نویسندگان
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