کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2072684 1544722 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inter- and intra-individual variability of total antioxidant capacity of bovine seminal plasma and relationships with sperm quality before and after cryopreservation
ترجمه فارسی عنوان
تنوع بین و درون فردی ظرفیت آنتی اکسیدانی بالقوه پلاسمای سمی گاو و روابط با کیفیت اسپرم قبل و بعد از گاو حفظ
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

The aim of the present study was to investigate inter- and intra-individual variability of total antioxidant capacity (TAC) in seminal plasma of bulls. In addition, relationships between TAC and glutathione peroxidase (GPx), superoxide dismutase activities (SOD), and parameters of sperm quality, respectively, were examined. Eight consecutive ejaculates were collected from nine Holstein–Friesian bulls. The percentage of plasma membrane and acrosome intact (PMAI) sperm was measured by using the FITC-PNA/PI assay, the amount of membrane lipid peroxidation (LPO) without and with stimulation(s) of LPO was quantified by using the BODIPY assay before cryopreservation and immediately (0 h) as well as 3 h after thawing. The percentage of sperm with a greater DNA fragmentation index was measured by the sperm chromatin structure assay. The amount of TAC differed (P < 0.0001) between bulls but not (P > 0.05) between ejaculates within bulls. The amounts of TAC were not related (P > 0.05) to amounts of SOD and GPx, but were negatively associated with LPO 0 h (r = −0.85; P ≤ 0.01). The amounts of SOD showed positive relationships with LPO 0 h (r = 0.71; P ≤ 0.05) and LPO 3 h (r = 0.80; P ≤ 0.05). In conclusion, total antioxidant activity varied among bulls, but not between ejaculates within bulls. While the amounts of antioxidative enzyme GPx was not related to sperm quality and SOD was positively related with lipid peroxidation after thawing of sperm, whereas total antioxidative capacity was negatively correlated with lipid peroxidation of cryopreserved sperm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 155, April 2015, Pages 99–105
نویسندگان
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