کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2072753 1544726 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic scavengers in the epididymal fluid: Comparison between pony and miniature breed stallions
ترجمه فارسی عنوان
پراکنشهای آنزیمی در مایع اپیدیدیم: مقایسهای میان ژولیدههای نژاد تپه و مینیاتوری
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


• Miniature breed males show a higher activity of catalase and superoxide dismutase in cauda epididymal fluid than pony stallions.
• Catalase and superoxide dismutase activities are higher in epididymal fluid from cauda.
• Activity of Glutathione peroxidase is equivalent in caput, corpus and cauda epididymal fluid of pony and miniature breed stallions.

The use of stallion semen collected from cauda epididymis for AI has increased due to the new protocols available for cryopreservation. Preserving the genetic material from valuable males that suffer sudden death or other events that prematurely end the stallion's reproductive life is an important strategy for Stud breeding management. While protecting spermatozoa from oxidative stress and infectious agents, the epididymis promotes the enhancement of sperm cell morphology and changes in membrane protein profile, increasing its fertility potential. The epididymal fluid must be a balanced redox environment to allow sperm preservation and protein–protein and protein–lipids interactions to quantify. The aim of this study was quantify the enzymatic ROS scavengers in epididymal fluid of pony and miniature breed stallions. Epididymides from 8 pony stallions and 12 miniature breed stallions were dissected and fluid from caput, corpus and cauda epididymis collected. Spermatozoa were separated of epididymal fluid by 2-step centrifugation. The activities of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured and compared between stallion groups and epididymal regions. The three enzymes were present in all epididymal regions tested, with higher activities of catalase and SOD in cauda epididymis in miniature breed stallions (P < 0.05). GPx activity was higher in caput epididymis in pony stallions (P < 0.05), however with no difference to fluid from cauda epididymis of both breeds. These results show a difference in antioxidant enzymatic scavengers between pony and miniature breed stallions. Also, our data confirm the protective role of cauda epididymis, preserving spermatozoa integrity from oxidative damage. As glutathione peroxidase is involved in several signaling pathways, its constant activity during epididymal transit corroborates the importance of this enzyme for spermatozoa maturation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 151, Issues 3–4, 30 December 2014, Pages 164–168
نویسندگان
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