کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2073258 1544755 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Longevity of chilled canine semen comparing different extenders
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Longevity of chilled canine semen comparing different extenders
چکیده انگلیسی

The use of chilled semen has raised increased interest due to several reasons. The present study compares 3 extenders for chilling of canine semen over a 10 days storage period. Sperm rich fractions (n = 30) were divided into three aliquots and diluted with (a) a self-made tris-egg yolk extender (TEY), (b) the commercial CaniPRO™Chill 10 (CP, Minitüb, Germany) and (c) the Uppsala Equex-2 system (UpA+B): UpA+B (n = 12) or UpA (n = 18). % of totally and progressively motile sperm using computer-assisted sperm analysis (CASA, TM, PM), % live, membrane intact spermatozoa (SYBR-14/propidium iodide), % morphologically abnormal sperm, % acrosomal changes (Spermac®) and % membrane intact sperm (HOS test) were examined on days 0, 1, 2, 3, 5, 7 and 10. Semen quality significantly decreased during storage with significantly lower motility and lower % of live, membrane intact spermatozoa in UpA+B diluted samples. Although quality of samples chilled with TEY, CP and UpA was similar within the first 3 days, only TEY and CP revealed good results in preserving semen quality over 10 days with significantly higher PM, CASA motility parameters and % morphologically normal sperm in CP-chilled samples (each p < 0.05).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 135, Issues 1–4, November 2012, Pages 97–105
نویسندگان
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