کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2073392 1544768 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequencing and gene expression of the porcine ITIH SSC13 cluster and its effect on litter size in an Iberian × Meishan F2 population
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Sequencing and gene expression of the porcine ITIH SSC13 cluster and its effect on litter size in an Iberian × Meishan F2 population
چکیده انگلیسی

The aim of the present study was to identify polymorphisms and to analyze endometrial gene expression of the porcine SSC13 ITIH cluster that could explain differences in prolificacy of 255 F2 sows derived from an Iberian (Ib) × Meishan (Me) intercross in which QTL for the number of piglets born alive (NBA) and total number of piglets born (TNB) were previously detected on this chromosome. Sequencing of ITIH-1, -3, and -4 mRNAs was done and several polymorphisms segregating within the Ib × Me population were found in all three genes. Significant associations with NBA were found for two SNPs from ITIH-1, four from ITIH-3, and four SNPs from ITIH-4 (p < 0.05). Haplotypes for the significant SNPs were calculated by segregation analysis and a marker assisted association test indicated that the alleles coming from the Meishan breed had a favorable effect on NBA for all three genes. Interestingly, some of the significant SNPs were located within the von Willebrand domain of the ITIH proteins, the binding site of molecules essential for the synthesis of the extracellular matrix during cumulus expansion. Gene expression analyses also revealed differences in the expression level of the ITIH-3 gene regarding the prolificacy performance (high or low) and the uterus sample (apical or basal).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 128, Issues 1–4, October 2011, Pages 85–92
نویسندگان
, , , , , ,