کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2074386 1544799 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Canine prostaglandin E2 synthase (PGES) and its receptors (EP2 and EP4): Expression in the corpus luteum during dioestrus
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Canine prostaglandin E2 synthase (PGES) and its receptors (EP2 and EP4): Expression in the corpus luteum during dioestrus
چکیده انگلیسی

In the dog CL are the only source of the progesterone in cyclic and pregnant animals. From a high expression of cyclooxygenase 2 (Cox2) at the beginning of the dioestrus and a low one at the end it was suggested that prostanoids may play a role in the formation of the CL. This led to the hypothesis that also in the dog PGE2 of luteal origin might act as paracrine/autocrine factor. Hence, expression of the prostaglandin E2 synthase (PGES) and its receptors (EP2 and EP4) was determined during the course of dioestrus in canine CL from days 5, 15, 25, 35, 45, 65 after ovulation, following cloning of PGES using SMART RACE PCR, which revealed a high homology (82–94%) with other species. Real Time (TaqMan) PCR showed a high PGES and EP2 expression in the early CL-phase with a significant decrease thereafter. EP4 revealed a constant expression pattern throughout the life span of the CL. In situ hybridization co-localized PGES, EP2 and EP4 in the cytoplasm of the luteal cells only. In conclusion, our data suggest that in the dog PGE2 of luteal origin acts by autocrine mechanism as a luteotropic factor through its EP2 and EP4 receptors during the phase of CL-formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 109, Issues 1–4, December 2008, Pages 319–329
نویسندگان
, , ,