Spermatozoal morphologies and fructose and citric acid concentrations in agouti (Dasyprocta leporina) semen

This study was conducted to identify the levels of fructose and citric acid, and sperm morphologies in agouti (Dasyprocta leporina) semen. These parameters may be important in identifying highly fertile semen from the agouti. The objectives were: (1) to investigate spermatozoal abnormalities in agouti semen and (2) to determine the concentrations of seminal fructose and citric acid in agouti semen samples. Semen samples were collected from 16 anaesthetised male agouti by electro-ejaculation. Fructose and citric acid concentrations were 256.86 ± 63.54 mg/dl and 1877 ± 147 mg/dl, respectively, measured with ELISA kits. Sperm morphologies, examined using eosin-negrosin staining, showed 11 morphologies. The most abundant (68.5%) sperm morphology (M1) showed no known sperm defects. Means for head, mid piece, tail and total length of the agouti spermatozoa was 5.23 ± 0.04 μm, 5.18 ± 0.08 μm, 37.52 ± 0.24 μm and 47.96 ± 0.25 μm, respectively for M1 sperm. The means of spermatozoa head and mid piece width and semen volume were 3.26 ± 0.04 μm, 0.70 ± 0.02 μm and 0.47 ± 0.16 ml, respectively. It was concluded that as the fructose concentration in agouti ejaculate increased the percentage of spermatozoa with known spermatozoa defects increased (r = 0.506; P < 0.037; n = 32). It is suggested that the M1 sperm could be the most competitive spermatozoa in agouti ejaculate. In conclusion standards for identifying fertile agouti semen were established.