کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2074428 1544809 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Production of superoxide anion and hydrogen peroxide by capacitating buffalo (Bubalus bubalis) spermatozoa
چکیده انگلیسی

In the present study attempts were made to detect and quantify the generation of superoxide anion (O2−) and hydrogen peroxide (H2O2) by capacitating buffalo spermatozoa. Ejaculated buffalo spermatozoa were suspended in sp-TALP medium at 50 × 106 mL−1 and incubated at 38.5 °C with 5% CO2 in air in the absence or presence of heparin (a capacitation inducer) or reduced nicotinamide adenine dinucleotide phosphate (NADPH) or diphenyleneiodonium (DPI, a flavoprotein inhibitor) for 6 h. Production rate of O2− and H2O2 by spermatozoa at different hours of capacitation were measured by cytochrome c reduction and phenol red oxidation assays, respectively. Spermatozoa generated both O2− and H2O2 spontaneously and following stimulation with heparin and a significant increase of O2− production was observed in the presence of NADPH. However, DPI inhibited this NADPH-induced O2− production and suggested for existence of putative NADPH-oxidase that constitute a specific O2− generating systems in buffalo spermatozoa. Results of our study indicated that buffalo spermatozoa generate O2− and H2O2 and production of these free radicals is induced during capacitation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 103, Issues 3–4, 30 January 2008, Pages 260–270
نویسندگان
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