کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2074578 | 1544813 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preservation of mithun (Bos frontalis) semen at refrigeration temperature
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The objective of the present study was to investigate the possibility of preserving mithun (Bos frontalis) spermatozoa at refrigeration temperature using tris-egg yolk diluent. Semen samples were collected from four adult mithun bulls through rectal massage method. Good quality semen samples (n = 30) were preserved at 4 °C using tris-egg yolk diluent for 72 h. Progressive motility, live spermatozoa count and morphological abnormalities were evaluated every 12 h until 72 h of preservation. The colour, consistency and mass activity of fresh semen samples were found to be creamy white, medium and 3+ to 4+ (5+ scale), respectively. The average (mean ± S.E.) volume (ml), pH and spermatozoa concentration (106 mlâ1) of fresh semen samples were found to be 0.6 ± 0.01, 6.8 ± 0.03 and 425 ± 48, respectively. Progressive motility and live spermatozoa count were found to be less than 30% (P < 0.01) after 48 h of storage. Head (P < 0.05), midpiece (P < 0.05), tail (P < 0.01) and total (P < 0.01) abnormalities were found to be increased significantly over the time of storage. It was observed that progressive motility and live spermatozoa count remained above 30% and 40%, respectively, until 36 h of storage. Simultaneously the percentage of morphologically abnormal spermatozoa was found to be significantly low until 36 h of storage. The results indicate that it is possible to preserve mithun spermatozoa at refrigeration temperature in tris-egg yolk diluent, which can be further used for artificial insemination within 36 h of storage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 101, Issues 3â4, October 2007, Pages 257-264
Journal: Animal Reproduction Science - Volume 101, Issues 3â4, October 2007, Pages 257-264
نویسندگان
M. Karunakaran, A. Dhali, A. Mech, K. Khate, C. Rajkhowa, D.P. Mishra,