کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2091862 | 1545982 | 2016 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Transcriptome sequencing analysis of novel sRNAs of Kineococcus radiotolerans in response to ionizing radiation Transcriptome sequencing analysis of novel sRNAs of Kineococcus radiotolerans in response to ionizing radiation](/preview/png/2091862.png)
Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results can identify these sRNAs in a genome-wide detection, using RNA sequencing (RNA-seq) by the deep sequencing technique. In this study, the raw data of radiation-exposed samples (RS) and control samples (CS) were acquired separately from the sequencing platform. There were 217 common sRNA candidates in the two samples screened in the genome-wide scale by bioinformatics analysis. There were 43 differentially expressed sRNA candidates, including 28 up-regulated and 15 down-regulated ones. The down-regulated sRNAs were selected for the sRNA target prediction, of which 12 sRNAs that may modulate the genes related to the transcription regulation and DNA repair were considered as the candidates involved in the radio-resistance regulation system.
Journal: Microbiological Research - Volume 192, November 2016, Pages 122–129