کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092722 1081811 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The microbial ecology of a high-temperature near-neutral spring situated in Rotorua, New Zealand
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
The microbial ecology of a high-temperature near-neutral spring situated in Rotorua, New Zealand
چکیده انگلیسی

SummaryA combination of both culture and culture-independent techniques were used to investigate the microbial ecology of a near-neutral, high-temperature hot spring (designated AQ1) in Rotorua, New Zealand. The active microbial members of the community were targeted by analyzing biofilms that developed on surfaces incubated in situ in AQ1. Colonization of surfaces was rapid as indicated by ATP assay and microscopic observation. DNA-based analysis of both colonized surfaces and pool water from AQ1 revealed an exclusively archaeal community. Different colonization patterns were observed on glass slides incubated near the pool surface or at depth. Slides incubated at the surface were colonized exclusively by Pyrobaculum species, while at greater depth a novel coccus was also observed and detected by DGGE. Sequence analysis revealed the coccus was related to Aeropyrum pernix. Two microorganisms were isolated from AQ1 pool water, namely Ignisphaera aggregans AQ1.S1T and a species of Pyrobaculum, isolate AQ1.S2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 163, Issue 5, 1 September 2008, Pages 594–603
نویسندگان
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