کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2092768 | 1081815 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and over-expression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and over-expression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli Identification and over-expression of a thermostable lipase from Geobacillus thermoleovorans Toshki in Escherichia coli](/preview/png/2092768.png)
چکیده انگلیسی
SummaryA newly isolated thermophilic strain producing thermostable lipase was identified based on 16S rRNA sequencing, where phylogenetic analysis revealed its closeness to Geobacillus thermoleovorans. Thermostable lipase from this bacterium was cloned using consensus degenerate PCR primers. For over-expression in Escherichia coli, the lipase gene was sub-cloned in pET 15b vector with a strong T7 promotor. Lipase activity was approximately 4.5-fold higher than in the wild-type strain. The lipase enzyme was thermostable at 60 °C and pH 8, whereas a 30% residual activity was retained when incubated for 1 h at 100 °C. Optimum lipase expression was obtained in 2×YT medium after 70 min of induction by IPTG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 163, Issue 1, 15 January 2008, Pages 13–20
Journal: Microbiological Research - Volume 163, Issue 1, 15 January 2008, Pages 13–20
نویسندگان
Yasser R. Abdel-Fattah, Ahmed A. Gaballa,