کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092848 1081821 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SigN is responsible for differentiation and stress responses based on comparative proteomic analyses of Streptomyces coelicolor wild-type and sigN deletion strains
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
SigN is responsible for differentiation and stress responses based on comparative proteomic analyses of Streptomyces coelicolor wild-type and sigN deletion strains
چکیده انگلیسی

SummaryTo address the functions of SigN (SCO4034) in Streptomyces coelicolor, we constructed a sigN null mutant M145Z, which showed a defect in sporulation. The differential proteomic profiles of wild-type S. coelicolors M145 and M145Z were demonstrated by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), which identified 24 different spots that were up- or down-regulated due to sigN disruption. Among them, 22 proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The up-regulated proteins were involved in energy metabolism, stress responses, ATP binding, ppGpp stringent stress response and transcriptional anti-termination, etc. The down-regulated proteins were related to antibiotic synthesis and differentiation. The results gave a new insight into the regulatory mechanism of SigN in S. coelicolor. Furthermore, deletion of sigN caused growth retardation under all stress conditions examined including heat, cold, acid, oxidation, salt and ethanol. These results indicated that SigN was involved in morphological development, secondary metabolism and stress responses in S. coelicolor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 165, Issue 3, 31 March 2010, Pages 221–231
نویسندگان
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