کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093158 1081928 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic study of cytokines production by human peripheral blood mononuclear cells in response to Brucella DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Kinetic study of cytokines production by human peripheral blood mononuclear cells in response to Brucella DNA
چکیده انگلیسی

SummaryIn spite of reports on cytokines induction by the Brucella DNA in murine model, there is no comparison between pathogenic and appropriate vaccine strains in human. We investigated the cytokines profile of human peripheral blood mononuclear cells (PBMCs) induced by DNA extracted from pathogenic isolates of Brucella melitensis and B. abortus as well as Rev1 and S19; the appropriate vaccine strains. It was observed that despite differential induction of Interleukin(IL)-12 and IL-10 production, identical IL-12/IL-10 concentration ratio was obtained by all Brucella strains DNAs that was 2 after 24 h and 4 after 5 days of incubation. In addition, IL-2 and Interferon(IFN)-γ production were profoundly increased compared to the medium at day 3 and 5 respectively but IFN-α was not induced. Therefore, Brucella strains DNAs are Th1 inducing component with similar pattern in human PBMCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 163, Issue 4, 15 July 2008, Pages 466–472
نویسندگان
, , , , , ,