کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2094254 1081997 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine
چکیده انگلیسی


• Differences between two anti-Lrig1 antibodies were characterized.
• A RFP-expressing Lrig1 reporter mouse, Lrig1-Apple.was generated.
• Anti-Lrig1 from R&D Systems™ appears to recognize all Lrig1+ cells.
• Anti-Lrig1-VU recognizes a subset of anti-Lrig1-R&D+ cells.

Lrig1 is an intestinal stem cell marker important for epithelial homeostasis. However, the position of the Lrig1+ population in the intestinal crypt has been debated, largely due to discrepant staining patterns using two Lrig1 antibodies. Here, we set out to decipher the differences between these Lrig1 antibodies to clarify their use for Lrig1-related studies. We confirmed that the commercially available Lrig1-R&D antibody stained the bottom third of the colonic crypt, whereas an independently generated Lrig1-VU antibody recognized a subset of anti-Lrig1-R&D+ cells. Biochemically, we found that anti-Lrig1-VU recognized a non-glycosylated form of Lrig1; in contrast, anti-Lrig1-R&D recognized both glycosylated and non-glycosylated forms of Lrig1. In addition, we generated a reporter mouse (Lrig1-Apple) as an independent readout of Lrig1 transcriptional activity. Flow cytometry of isolated colonic epithelial cells from Lrig1-Apple mice demonstrated anti-Lrig1-R&D recognized mostly RFP-hi cells, while anti-Lrig1-VU recognized cells that were largely RFP-mid. Of note, by qRT-PCR, Lgr5 was expressed in the RFP-hi population, but not in the RFP-mid population. We conclude that anti-Lrig1-R&D appears to recognize all Lrig1+ cells, while anti-Lrig1-VU recognizes a subpopulation of Lrig1+ cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 13, Issue 3, Part A, November 2014, Pages 422–430
نویسندگان
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