کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2094535 | 1082026 | 2014 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Lactate regulates myogenesis in C2C12 myoblasts in vitro Lactate regulates myogenesis in C2C12 myoblasts in vitro](/preview/png/2094535.png)
• Lactate enhances the serum-withdrawal induced cell cycle arrest in myoblasts.
• Lactate promotes early differentiation in myoblasts.
• Lactate delays late differentiation in myoblasts in a timely manner.
• Lactate increases the generation of reactive oxygen species (ROS).
• Lactate effects can be reversed by the addition of antioxidants.
Satellite cells (SCs) are the resident stem cells of skeletal muscle tissue which play a major role in muscle adaptation, e.g. as a response to physical training. The aim of this study was to examine the effects of an intermittent lactate (La) treatment on the proliferation and differentiation of C2C12 myoblasts, simulating a microcycle of high intensity endurance training. Furthermore, the involvement of reactive oxygen species (ROS) in this context was examined. C2C12 myoblasts were therefore repeatedly incubated for 2 h each day with 10 mM or 20 mM La differentiation medium (DM) and in some cases 20 mM La DM plus different antioxidative substances for up to 5 days. La free (0 mM) DM served as a control. Immunocytochemical staining, Western blot analysis and colorimetric assays were used to assess oxidative stress, proliferation, and differentiation. Results show that La induces oxidative stress, enhances cell-cycle withdrawal, and initiates early differentiation but delays late differentiation in a timely and dose-dependent manner. These effects can be reversed by the addition of antioxidants to the La DM. We therefore conclude that La has a regulatory role in C2C12 myogenesis via a ROS-sensitive mechanism which elicits implications for reassessing some aspects of training and the use of nutritional supplements.
Journal: Stem Cell Research - Volume 12, Issue 3, May 2014, Pages 742–753