کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2112409 | 1084382 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein Targeted killing of rhabdomyosarcoma cells by a MAP-based human cytolytic fusion protein](/preview/png/2112409.png)
چکیده انگلیسی
The treatment of rhabdomyosarcoma (RMS) is challenging, and the prognosis remains especially poor for high-grade RMS with metastasis. The conventional treatment of RMS is based on multi-agent chemotherapy combined with resection and radiotherapy, which are often marked by low success rate. Alternative therapeutic options include the combination of standard treatments with immunotherapy. We generated a microtubule-associated protein (MAP)-based fully human cytolytic fusion protein (hCFP) targeting the fetal acetylcholine receptor, which is expressed on RMS cells. We were able to express and purify functional scFv35-MAP from Escherichia coli cells. Moreover, we found that scFv35-MAP is rapidly internalized by target cells after binding its receptor, and exhibits specific cytotoxicity toward FL-OH1 and RD cells in vitro. We also confirmed that scFv35-MAP induces apoptosis in FL-OH1 and RD cells. The in vivo potential of scFv35-MAP will need to be considered in further studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 365, Issue 2, 1 September 2015, Pages 149-155
Journal: Cancer Letters - Volume 365, Issue 2, 1 September 2015, Pages 149-155
نویسندگان
Hannes Brehm, Dmitrij Hristodorov, Alessa Pardo, Radoslav Mladenov, Judith Niesen, Rainer Fischer, Mehmet K. Tur, Stefan Barth,