کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2113070 1084439 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CHC promotes tumor growth and angiogenesis through regulation of HIF-1α and VEGF signaling
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
CHC promotes tumor growth and angiogenesis through regulation of HIF-1α and VEGF signaling
چکیده انگلیسی

Pancreatic adenocarcinoma is an aggressive disease with a high mortality rate. In this study, we have newly generated a monoclonal antibody (mAb), Pa65-2, which specifically binds to pancreatic cancer cells and tumor blood vessels. The target protein of Pa65-2 is identified as human clathrin heavy chain (CHC). In vitro and In vivo study showed that suppression of CHC either by shRNA or by Pa65-2 inhibited tumor growth and angiogenesis. One of the key functions of CHC was to bind with the hypoxia-inducing factor (HIF)-1α protein, increasing the stability of this protein and facilitating its nuclear translocation, thereby regulating the expression of VEGF. Taken together, our findings indicate that CHC plays a role in the processes of tumorigenesis and angiogenesis. Pa65-2 antibody or CHC shRNA can potentially be used for pancreatic cancer therapy.


► CHC promotes tumor growth, invasion and angiogenesis in vitro and in vivo.
► CHC interacts with and stabilizes HIF-1α, and leads to an increased production of VEGF.
► Pa65-2 antibody can potentially be used to inhibit tumor angiogenesis and growth.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 331, Issue 1, 30 April 2013, Pages 58–67
نویسندگان
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