Nuclear receptor mRNA expression by HBV in human hepatoblastoma cell lines

Recent studies have implicated nuclear receptors (NRs) in the development of hepatocarcinogenesis. We assumed that hepatitis B virus (HBV) alters the expression of NRs and coregulators, and compared the gene expression profiling for 84 NRs and related genes between HpeG2.2.15, which secretes complete HBV virion, and HepG2 by real-time RT-PCR with SyBr green. Forty (47.6%) genes were upregulated 2-fold or greater, and only 5 (5.9%) were downregulated 2-fold or more, in HepG2.2.15 compared to HepG2. These results suggest that HBV affects NRs and their related signal transduction, and that they play important roles in viral replication and HBV-related hepatocarcinogenesis.

► Nuclear receptor-signal pathways in HepG2.2.15 are different from those in HepG2.
► HBV affects nuclear receptors and their related signal transduction.
► We focused on androgen receptor and histone deacetylase among nuclear receptors.
► Nuclear receptors play important roles in viral replication and hepatocarcinogenesis.