کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2113685 1084487 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response
چکیده انگلیسی

A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 309, Issue 2, 28 October 2011, Pages 170–175
نویسندگان
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