کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2113687 | 1084487 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preoperative analysis of 11q loss using circulating tumor-released DNA in serum: A novel diagnostic tool for therapy stratification of neuroblastoma Preoperative analysis of 11q loss using circulating tumor-released DNA in serum: A novel diagnostic tool for therapy stratification of neuroblastoma](/preview/png/2113687.png)
Allelic deletion of the long arm of chromosome 11 (11q loss) is closely associated with the prognosis of neuroblastoma (NB). Here we examined 11q loss using tumor-released DNA fragments in the sera of 24 cases. The allelic intensity score of a panel of polymorphic markers in 11q23 in serum DNA was significantly different between the 11q loss-positive group and the11q loss-negative group. The 11q loss-positive and -negative groups did not overlap when a cut-off value of 0.5 was chosen for the allelic intensity score. Our serum-based 11q loss analysis could predict the allelic status of 11q in tumors.
► We established a serum-based assay system for detecting 11q loss in neuroblastoma (NB).
► Microsatellite analysis was performed to detect the 11q status using serum DNA.
► Our 11q loss analysis completely matched the results of the MLPA analysis using the tumor DNA.
► Preoperative information of 11q status can provide appropriate therapeutic strategy in NB.
Journal: Cancer Letters - Volume 309, Issue 2, 28 October 2011, Pages 185–189