کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2115270 1084584 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN
چکیده انگلیسی

PAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27Kip1. These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 253, Issue 2, 18 August 2007, Pages 215–223
نویسندگان
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