Inhibition of gastric cancer cells associated angiogenesis by 15d-prostaglandin J2 through the downregulation of angiopoietin-1

Peroxisome proliferator-activated receptor gamma (PPARγ) ligands have been shown to inhibit angiogenesis. We showed that treatment with 15d-PGJ2, a PPARγ ligand, downregulate the expressions of angiopoietin-1 (Ang-1) in gastric cancer cells MKN45. The medium of MKN45 cells treated with 15d-PGJ2 significantly inhibited the migration and tube formation of human umbilical vein endothelial cells (HUVECs). Moreover, Matrigel plug assay revealed that 15d-PGJ2 reduced in vivo angiogenesis induced by MKN45 cells. These modulations were restored by the addition of recombinant Ang-1. Our findings supported that 15d-PGJ2 suppressed angiogenesis of gastric cancer cells by downregulation of Ang-1.