کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2115564 1546702 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methylation status of the Ep-CAM promoter region in human breast cancer cell lines and breast cancer tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Methylation status of the Ep-CAM promoter region in human breast cancer cell lines and breast cancer tissue
چکیده انگلیسی

We examined the methylation status of the Ep-CAM promoter region of human breast cancer cell lines and breast cancer tissue using MethyLight technology and bisulfite sequencing. We found the promoter of Ep-CAM-negative breast cancer cell lines Hs 578T to be methylated to a higher degree as compared to positive cell lines MCF-7. Demethylation of cell lines was performed using 5-aza-2′-deoxycytidine. Ep-CAM RNA and protein expression could be partially restored by treating cells with 5-Aza-2′-deoxycytidine. In most primary breast cancer tissue, methylation of the Ep-CAM gene could be detected at a low level and no correlation was found with Ep-CAM protein expression in tumour tissue. Taken together, these data suggest that methylation of the Ep-CAM promoter is not a crucial mechanism for regulation of Ep-CAM expression in breast cancer. Thus, most important regulatory mechanisms have to be supposed in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 246, Issues 1–2, 8 February 2007, Pages 253–261
نویسندگان
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