کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2116037 1084622 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemosensitization of bladder cancer cells by survivin-directed antisense oligodeoxynucleotides and siRNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Chemosensitization of bladder cancer cells by survivin-directed antisense oligodeoxynucleotides and siRNA
چکیده انگلیسی

Survivin is known to be overexpressed in numerous tumor types including human bladder cancer and to cause resistance to radiation and chemotherapy. Therefore, we tested the antisense oligodeoxynucleotide AS-SVV286 and the small interfering RNA si-SVV284 to down-regulate survivin in the BCa cell lines EJ28 and 5637 thereby acting as sensitizers for chemotherapy. Pretreatment with these inhibitors followed by chemotherapy caused an enhanced decrease in cell viability. The observed reduction in cell counts associated with increased rates of apoptosis paralleled the degree of reduction of survivin expression that was achieved more efficiently by the siRNA than by the AS-ODN. Nevertheless, both therapy approaches in combination with all tested chemotherapeutics provoked a remarkable inhibition of viability and may serve as suitable additive tools for chemosensitization of bladder cancer cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 232, Issue 2, 8 February 2006, Pages 243–254
نویسندگان
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