کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2116758 1085028 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of CpG island methylation in progressive breast lesions from normal to invasive carcinoma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Quantification of CpG island methylation in progressive breast lesions from normal to invasive carcinoma
چکیده انگلیسی

Epigenetic silencing of specific genes is associated with cancer progression. CpG islands are present at higher frequency in promoter regions, their methylation leading to gene underexpression. Pyrosequencing provides sequencing analysis of genetic markers, e.g., single nucleotide polymorphisms and DNA methylation. We investigated methylation levels of a spectrum of neoplastic breast lesions, ranging from hyperplasia to invasive carcinoma obtained from the same patient. Assays were designed to analyze promoter regions of RASSF1A, GSTP1, RARβ, and E-cadherin. Methylation increased from normal to hyperplasia, the increase being significantly higher in invasive and in situ tumors for RASSF1A (p = 0.00006 and p = 0.009, respectively).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 257, Issue 1, 8 November 2007, Pages 136–144
نویسندگان
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