کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2116798 | 1085033 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: 14-3-3 sigma increases the transcriptional activity of the androgen receptor in the absence of androgens 14-3-3 sigma increases the transcriptional activity of the androgen receptor in the absence of androgens](/preview/png/2116798.png)
The androgen receptor (AR) is a ligand-activated transcription factor that regulates numerous target genes, including prostate-specific antigen (PSA). We examined the ability of each member of the 14-3-3 family to modulate transcription of PSA through the AR. Despite significant homology within the 14-3-3 family we observed differences in the ability of each isoform to alter the transcriptional activity of the AR. Significantly, 14-3-3 sigma activated PSA-luciferase reporters not only at castrate levels of androgens, but also in the complete absence of androgens. 14-3-3 sigma also increased expression of the endogenous PSA gene in the absence of androgens. Knockdown of the AR by siRNA oligonucleotides abolished activation of these reporters by 14-3-3 sigma. These findings may have greatest significance in hormone refractory prostate cancer where the AR may be activated in a ligand-independent manner.
Journal: Cancer Letters - Volume 254, Issue 1, 28 August 2007, Pages 137–145