کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2119334 1546794 2014 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Decellularized ECM effects on human mesenchymal stem cell stemness and differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Decellularized ECM effects on human mesenchymal stem cell stemness and differentiation
چکیده انگلیسی


• Several cell type ECMs can be cold-EDTA decellularized (dcECM) with minimal damage.
• hMSC dcECM enhances naïve hMSC proliferation and maintenance of stemness.
• Osteogenic hMSC dcECM induces hMSC differentiation in the absence of dexamethasone.
• Smooth muscle cell dcECMs induce hMSC differentiation into smooth muscle-like cells.

Microenvironment extracellular matrices (ECMs) influence cell adhesion, proliferation and differentiation. The ECMs of different microenvironments have distinctive compositions and architectures. This investigation addresses effects ECMs deposited by a variety of cell types and decellularized with a cold-EDTA protocol have on multipotent human mesenchymal stromal/stem cell (hMSC) behavior and differentiation. The cold-EDTA protocol removes intact cells from ECM, with minimal ECM damage and contamination. The decellularized ECMs deposited by cultured hMSCs, osteogenic hMSCs, and two smooth muscle cell (SMC) lines were tested for distinctive effects on the behavior and differentiation of early passage (‘naïve’) hMSC plated and cultured on the decellularized ECMs. Uninduced hMSC decellularized ECM enhanced naïve hMSC proliferation and cell motility while maintaining stemness. Decellularized ECM deposited by osteogenic hMSCs early in the differentiation process stimulated naïve hMSCs osteogenesis and substrate biomineralization in the absence of added dexamethasone, but this osteogenic induction potential was lower in ECMs decellularized later in the osteogenic hMSC differentiation process. Decellularized ECMs deposited by two smooth muscle cell lines induced naïve hMSCs to become smooth muscle cell-like with distinctive phenotypic characteristics of contractile and synthetic smooth muscle cells. This investigation demonstrates a useful approach for obtaining functional cell-deposited ECM and highlights the importance of ECM specificity in influencing stem cell behavior.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 88, Issues 4–5, November–December 2014, Pages 131–143
نویسندگان
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