کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2119547 1546803 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PKC-δ signalling pathway is involved in H9c2 cells differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
PKC-δ signalling pathway is involved in H9c2 cells differentiation
چکیده انگلیسی

H9c2 are rat heart embryonic myoblasts, with skeletal muscle properties, which terminally differentiate by fusing and forming multinucleated myotubes. Here we investigated the possible involvement of Protein Kinases C (PKCs) in H9c2 cell differentiation and explored the interplay of these enzymes both with reactive oxygen species (ROS), upstream physiological mediators of cell differentiation, and with nitric oxide (NO), downstream target of PKC activation, known for being involved in apoptosis induction in differentiated myoblasts. Cells were induced to differentiate (6 days) under low serum culture conditions and assayed for the expression of cell cycle (cyclin A) and differentiation markers (morphology and myogenin). Both ROS and in vivo production of NO were found increased after 6 days of differentiation, when the activation of PKC-δ isoform was 14-fold increased compared with the undifferentiated control cells. The parallel analysis of apoptotic features demonstrated a small increase in Annexin-V+ cells and a concomitant increase in PARP cleavage and Bax expression. Interestingly, a reduced percentage of differentiated cells was obtained both in the presence of Rottlerin, a highly selective PKC-δ pharmacologic inhibitor, and, moreover, with the use of PKC-δ siRNA technology, further supporting the involvement of PKC-δ in switching on the events related to skeletal muscle myoblast differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 80, Issues 4–5, November–December 2010, Pages 204–212
نویسندگان
, , , , , , ,