کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2119572 1085402 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED)
چکیده انگلیسی

Stem cell fate can be induced by the grade of stiffness of the extracellular matrix, depending on the developed tissue or complex tissues. For example, a rigid extracellular matrix induces the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs), while a softer surface induces the osteogenic differentiation in dental follicle cells (DFCs). To determine whether differentiation of ectomesenchymal dental precursor cells is supported by similar grades of extracellular matrices (ECMs) stiffness, we examined the influence of the surface stiffness on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED). Cell proliferation of SHED was significantly decreased on cell culture surfaces with a muscle-like stiffness. A dexamethasone-based differentiation medium induced the osteogenic differentiation of SHED on substrates of varying mechanical stiffness. Here, the hardest surface improved the induction of osteogenic differentiation in comparison to that with the softest stiffness. In conclusion, our study showed that the osteogenic differentiation of ectomesenchymal dental precursor cells SHED and DFCs are not supported by similar grades of ECM stiffness.


► Soft stiffness supports osteogenic differentiation in dental follicle cells (DFC).
► Relation stiffness & differentiation in deciduous teeth stem cells (SHED) is unknown.
► Our study examined stiffness & differentiation of SHED.
► rigid ECMs have a superior capacity to support the osteogenic differentiation of SHED.
► DFCs & SHED react contrarily to soft and rigid surface stiffness.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 84, Issue 5, December 2012, Pages 366–370
نویسندگان
, , , , , , , , ,