کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2120144 1085445 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative analysis of Xenopus VegT, the meso-endodermal determinant, identifies an unusual conserved sequence
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Comparative analysis of Xenopus VegT, the meso-endodermal determinant, identifies an unusual conserved sequence
چکیده انگلیسی

The transcription factor, VegT, is the meso-endodermal determinant in Xenopus laevis. We examined VegT orthologs from several anuran amphibians and the urodele amphibian, the Mexican axolotl. In addition to the conserved T-box, the DNA-binding domain, the orthologs share a conserved 57 amino acid domain at the C-terminal. Most striking is a 17-nucleotide (nt) sequence near the 3′ end of the open reading frame. The 17 nts are absolutely conserved among the anurans, whose last common ancestor lived 200 million years ago. As an initial test of the function of the 17 nts, 27 or 49 amino acids, which include the six amino acids coded by the 17 (+1) nts, were deleted from the C-terminal of VegT. These truncated VegT's retained some transcriptional activity, indicating that the 17 nts are not absolutely required for this function. The function of the highly conserved 17 nts is unknown. Two possibilities are that the conserved 17 nts interact with the cytoskeleton or that they are a target for regulation by a microRNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 75, Issue 6, July 2007, Pages 559–565
نویسندگان
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