کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2121356 1546897 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measles Epidemics Among Children in Vietnam: Genomic Characterization of Virus Responsible for Measles Outbreak in Ho Chi Minh City, 2014
ترجمه فارسی عنوان
اپیدمی سرخک در بین کودکان در ویتنام: تشخیص ژنوم ویروس مسئول شیوع سرخک در شهر هوشی مین، 2014
کلمات کلیدی
سرخک ویروس سرخک ژنوتیپ ویروس، ویتنام، جنوب شرقی آسیا
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


• Measles viruses responsible for outbreaks among children in Southern Vietnam in 2014, belonged to genotype D8 variant group which had unique amino acid sequences in the N-450 region of the N gene.
• Our report provides important genomic information of the virus for measles elimination in Southeast Asia.

BackgroundMeasles remains poorly controlled in Southeast Asia, including Vietnam.ObjectivesThe aim of this study was to characterize genes of virus responsible for a measles outbreak among children in Vietnam in 2014.Study designThroat swab samples were collected from 122 pediatric patients with suspected measles. Furthermore, peripheral blood mononuclear cells (PBMCs) from 31 of these cases were also collected. Measles virus (MV) RNA was obtained directly from the clinical specimens, amplified by PCR, and then the N and H genes were sequenced.ResultsMV RNA was detectable in throat swabs from all 122 patients tested. Positive-strand viral RNA, which indicates the intermediate replicative form of MV, was also detected in PBMCs from all 31 cases from whom these cells were collected. One hundred and eighteen strains with the N gene were obtained by RT-PCR and sequenced. Using phylogenetic analysis with measles reference sequences, all of the Vietnamese strains were found to be genotype D8. However, all strains formed a distinct cluster within the genotype D8 group (D8-VNM) suggesting their own lineage. This distinct cluster was supported by a branch with a 99% bootstrap value and 3.3% nucleotide divergence in the N-450 region of the N gene from the D8 reference strain. Notably, all of the D8-VNM variant strains represented unique amino acid sequences consisting of R442, S451 and G452 in the N-450 region of the N gene.ConclusionsMeasles viruses responsible for outbreaks in Southern Vietnam belonged to a genotype D8 variant group which had unique amino acid sequences in the N gene. Our report provides important genomic information about the virus for measles elimination in Southeast Asia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: EBioMedicine - Volume 1, Issues 2–3, December 2014, Pages 133–140
نویسندگان
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