کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2136830 1087813 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a novel treatment for leukemia directed at tumor-associated mRNA splicing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Development of a novel treatment for leukemia directed at tumor-associated mRNA splicing
چکیده انگلیسی

This report describes a novel approach to cancer therapy that targets genes that are preferentially alternatively spliced and expressed in leukemia. We developed CD44v6 and CD44v8 splicing constructs fused with GFP or a humanized fragment of Pseudomonas aeruginosa exotoxin A (hPE24). Transfection of K562 leukemia cells with the GFP-linked splicing constructs led to subsequent production of detectable levels of GFP. Transfection of K562 cells with the hPE24-linked splicing constructs led to significant reduction of cell viability and an increase in the induction of apoptosis. Normal human PBMCs were unaffected by following transfection with these constructs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Leukemia Research - Volume 37, Issue 9, September 2013, Pages 1125–1131
نویسندگان
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