کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2144779 1548019 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thrombospondin-1 and CD47 signaling regulate healing of thermal injury in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Thrombospondin-1 and CD47 signaling regulate healing of thermal injury in mice
چکیده انگلیسی


• Absence of CD47 improves the rate of wound closure for a focal dermal thermal injury.
• Thrombospondin-1 and TGF-β1 signaling are upregulated in CD47 deficient wounded tissue.
• An early increase in collagen deposition is observed in CD47 deficient wounded tissue.
• CD47 deficiency regulates inflammatory response and vascularization to improve wound healing.

More than 2.5 million Americans suffer from burn injuries annually, and burn management is a major public health problem. Treatments have been developed to manage wound injuries employing skin grafts, various dressings and topical and systemic agents. However, these often achieve limited degrees of success. We previously reported that targeting the interaction of thrombospondin-1 with its signaling receptor CD47 or deletion of the genes encoding either of these proteins in mice improves recovery from soft tissue ischemic injuries as well as tissue injuries caused by ionizing radiation. We now demonstrate that the absence of CD47 improves the rate of wound closure for a focal dermal second-degree thermal injury, whereas lack of thrombospondin-1 initially delays wound closure compared to healing in wild type mice. Doppler analysis of the wounded area showed increased blood flow in both CD47 and thrombospondin-1 null mice. Accelerated wound closure in the CD47 null mice was associated with increased fibrosis as demonstrated by a 4-fold increase in collagen fraction. Wound tissue of CD47 null mice showed increased thrombospondin-1 mRNA and protein expression and TGF-β1 mRNA levels. Activation of latent TGF-β1 was increased in thermally injured CD47-null tissue as assessed by phosphorylation of the TGF-β1 receptor-regulated transcription factors SMAD-2 and -3. Overall these results indicate that targeting CD47 may improve the speed of healing thermal injuries, but some level of CD47 expression may be required to limit the long term TGF-β1-dependent fibrosis of these wounds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 37, July 2014, Pages 25–34
نویسندگان
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