Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model

► Splice site targeting strategy inhibited type II procollagen alternative splicing. ► Knock-in mice synthesizing only type IIA procollagen were generated. ► IIA procollagen knock-in mice are viable with no apparent overt phenotype. ► Post-natal cartilage analysis in IIA knock-in mice currently in progress.