Genomic imbalances in endometrial adenocarcinomas – Comparison of DNA ploidy, karyotyping and comparative genomic hybridization

DNA ploidy analysis is useful for prognostication in cancer patients, but the genomic details underlying ploidy changes are not fully understood. To improve this understanding, we compared DNA ploidy status with karyotypic and comparative genomic hybridization data on 51 endometrial adenocarcinomas. Out of 34 DNA diploid tumors evaluated by CGH, 16 (47%) showed imbalances, though only two had more than four copy number changes. Ten (29%) had aberrations involving chromosome 1, seven (21%) involving chromosome 10, while one tumor had a chromosome 8 aberration. Four of the seven DNA tetraploid tumors (57%) had imbalances detected by CGH with two (29%) having more than four. Six out of eight DNA aneuploid tumors showed imbalances by CGH, with five (63%) having more than four. The aberrations were observed on chromosomes 1 and 8 in five/eight (63%) cases while four imbalances (50%) involved chromosomes 5, 7 and X. Not surprisingly, we observed a significant correlation between increasing DNA ploidy complexity and increasing number of copy alterations. Gains of material from chromosomes 8 and 7 might be specifically correlated to DNA aneuploidy in endometrial adenocarcinomas since 63% and 50% of the aneuploid compared to 3% of the diploid tumors showed imbalances involving these chromosomes.

► Reveal specific CGH aberrations related to DNA aneuploidy in endometrial carcinomas.
► Correlation between increasing DNA ploidy complexity and copy alterations.
► 63% of the DNA aneuploid lesions had aberrations on chromosome 8.
►  50% of the DNA aneuploid lesions had aberrations on chromosome 7.
► Only 3% of the DNA diploid lesions had aberrations on chromosome 7 or 8.