کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2146072 1088849 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
E2-mediated cathepsin D (CTSD) activation involves looping of distal enhancer elements
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
E2-mediated cathepsin D (CTSD) activation involves looping of distal enhancer elements
چکیده انگلیسی

Estrogen receptor alpha (ERα) is a ligand dependent transcription factor that regulates the expression of target genes through interacting with cis-acting estrogen response elements (EREs). However, only a minority of ERα binding sites are located within the proximal promoter regions of responsive genes. Here we report the characterization of an ERE located 9 kbp upstream of the TSS of the cathepsin D gene (CTSD) that up-regulates CTSD expression upon estrogen stimulation in MCF-7 cells. Using ChIP, we show recruitment of ERα and phosphorylated PolII at the CTSD distal enhancer region. Moreover, we determine the kinetics of transient CpG methylation on the promoter region of CTSD and for the first time, at a distal enhancer element. We show that ERα is crucial for long-distance regulation of CTSD expression involving a looping mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Oncology - Volume 2, Issue 2, August 2008, Pages 182–190
نویسندگان
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